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THE CHARACTERISTICS OF DISTRIBUTION OF TYPES OF DENGUE VIRUS SERUM AND LEVEL OF CONSENSUS RESULTS AGREEMENT ON DIAGNOSTIC TESTING FOR DENGUE HEMORRHAGIC FEVER IN PEOPLE WITH FEVER OF QUANG NINH PROVINCE IN 2024
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Objectives: Characteristics of the Dengue virus serotypes by Realtime PCR method in Dengue hemorrhagic fever patients and comparing the similarity between the results of the Dengue virus serotype testing using the Realtime PCR method with the NS1 rapid test technique on Dengue hemorrhagic fever patients in Quang Ninh Province from 2022 - 2023.
Subjects and methods: A retrospective study of the statistical reports on 300 patients suspected of having
Dengue fever were collected at the Center for Disease Control in Quang Ninh Province from 2022 - 2023,
cross-sectional study.
Results: Patients infected with DENV1; 2; 4 accounted for 59.1%, 39.1%, 1.7%, respectively. The distribution of DENV1 type by region, DENV1 in the central region (Ha Long, Cam Pha) (46%) and western region (Uong Bi, Dong Trieu, Quang Yen) (41%) predominates over the eastern region and island districts; DENV2 type is higher in the western region (39.1%) than the central region (33.7%). The distribution of DENV types by subjects (≤ 15 years old, > 15 years old, man and women) and by time, clinical grade, and occupational group did not differ. Overall sensitivity and specificity of the rapid test were 93.19% and 96.92% with 95% CI. Positive predictive value (PPV) is: 99.09%, negative predictive value (NPV) is: 79.74% with 95% CI. The positive rate of rapid test was found to be higher at cut-off period value ≤ 25. The similarity calculated by Cohen's Kappa coefficient was 83.6%.
Conclusions: Three types of DENV were detected, of which DENV1 had the highest rate (59.1%) followed by DENV 2 (39.1%), DENV 4 accounted for a very low rate (1.7%). The NS1 rapid antigen test can be used as a very good screening test to identify Dengue virus infection status
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Từ khóa
Dengue virus, DENV serotypes, NS1 Rapid antigen detection, Realtime PCR typing
Tài liệu tham khảo
2. Andries AC, Duong V, Ngan C, Ong S, Huy R, Sroin KK, Te V, Y B, Try PL, Buchy P. Field evaluation and impact on clinical management of a
rapid diagnostic kit that detects Dengue NS1, IgM and IgG. PLoS Negl Trop Dis. 2012; 6:e1993.
3. Ministry of Health. Circular 54/2015/TT-BYT on the latest regime of information on reporting and declaration of infectious diseases,
(2015).
4. Nguyen Manh Hung, Tran Thi Ngoc Anh, Nguyen Thi Kieu Anh. Circulation and some epidemiological characteristics of Dengue virus
types causing Dengue fever in Hanoi, period 2015-2017. Journal of Preventive Medicine. 2018; 28(5):88-96.
5. Epidemiological characteristics of serotypes of Dengue virus causing Dengue hemorrhagic fever in some districts of Hanoi (2017-2019).
Journal of 108 - Clinical Medicine and Phamarcy. 2023; 18(3):56-60. https://doi.org/10.52389/ydls. v18i3.1810.
6. Van NTT, Nga PTT, Tuan LV, Trung NV, Quan LDM, Chien VC. Circulation of Dengue virus serotypes causing Dengue fever in the Central Highlands region in the period 2003 - 2020. Journal of Preventive Learning. 24/5/2022; 32(2):64-9.
https://doi.org/10.51403/0868-2836/2022/609.
7. Sasmono RT, Wahid I, Trimarsanto H, Yohan B, Wahyuni S, Hertanto M, và c.s. Genomic analysis and growth characteristic of Dengue viruses from Makassar, Indonesia. Infect Genet Evol. 2015;32:165-77.
8. Ariyaratne D, Gomes L, Jayadas TTP, Kuruppu H, Kodituwakku L, Jeewandara C, et al. (2022) Epidemiological and virological factors determining Dengue transmission in Sri Lanka during the COVID-19 pandemic. PLOS Glob Public Health 2(8): e0000399. https://doi.
org/10.1371/journal.pgph.0000399.
9. Ha Quoc Viet, Truong Ngoc Phuoc. Survey of the association between clinical and subclinical with Dengue virus type and evaluation of treatment results of severe Dengue hemorrhagic fever in children. Can Tho Journal of Medicine and Pharmacy. 2020; 1(29):110-116.
10. Sharma S, Tandel K, Danwe S, Bhatt P, Dash PK, Ranjan P, và c.s. Simultaneous detection and serotyping of Dengue infection using single
tube multiplex CDC Dengue Real-Time RT-PCR from India. Virusdisease.
11. Binh DN, Cuong HX, Thinh TTH, Thuy VTB, Son TT, Tien PH. Application of mAbNS1- DENV1-4 monoclonal antibody to detect NS1
antigen of Dengue virus types by ELISA method.jmpm. 1/3/2024; 49(2):174-85.
12. Nguyen Minh Ha, Nguyen Quang Huy, Le Thi Thu Van, Pham Hung Van. The clinical diagnostic value of the rapid test for the detection
of NS1 antigen and the rapid test for the detection of Dengue virus-specific Igm antibodies in the plasma of people with Dengue hemorrhagic fever. Vietnam Medical Journal. 2023; 529(2):267-271.
13. Chong ZL, Sekaran SD, Soe HJ, Peramalah D, Rampal S, Ng CW. Diagnostic accuracy and utility of three Dengue diagnostic tests for the
diagnosis of acute Dengue infection in Malaysia. BMC Infect Dis. 12/3/2020;20:210.
14. Ty Hang V, Minh Nguyet N, The Trung D, Tricou V, Yoksan S, Minh Dung N, và c.s. Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid
Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses. PLoS Negl Trop Dis. 2009;3(1):e360. https://doi.
org/10.1371/journal.pntd.0000360.
15. Huhtamo E, Hasu E, Uzcátegui NY, Erra E, Nikkari S, Kantele A, và c.s. Early diagnosis of Dengue in travelers: Comparison of a novel realtime RT-PCR, NS1 antigen detection and serology. J Clin Virol. 2010;47(1):49-53