THE VALUE OF ELISA METHOD FOR MEASURING ANTI-VINCULIN ANTIBODIES IN THE DIAGNOSIS OF BLEEDING ULCERATIVE COLITIS AND DIARRHEA-PREDOMINANT IRRITABLE BOWEL SYNDROME AT THANH NHAN HOSPITAL IN 2023

Tóm tắt

Objectives: To evaluate the diagnostic value of the ELISA method for measuring anti-vinculin antibodies in the diagnosis of bleeding ulcerative colitis (UC) and diarrhea-predominant irritable bowel syndrome (IBS-D) at Thanh Nhan Hospital in 2023.
Subjects and methods: This was a cross-sectional study conducted with 150 participants, including 30 healthy individuals, 50 patients with UC, and 70 patients with IBS-D. The patients were examined and treated at Thanh Nhan Hospital from January 2023 to June 2024.
Results: The average duration of disease for patients with UC and IBS-D was 2.8 ± 2.0 years and 2.9 ± 4.7 years, respectively. The average age of UC and IBS-D patients was 46.8 ± 13.7 years and 48.4 ± 12.0 years, respectively. The average level of anti-vinculin antibodies in the UC and IBS-D groups was 372.0
± 196.1 (µg/g) and 587.7 ± 315.7 (µg/g), respectively. With an anti-vinculin antibody threshold in serum of 535.8 ng/mL (1.8 OD), the diagnostic value for distinguishing IBS-D from healthy individuals had a sensitivity of 50% and a specificity of 87.5%.
Conclusions: The level of anti-vinculin antibodies in the IBS-D group (539.2 ± 299.3 ng/mL) was significantly higher than in the healthy control group (345.8 ± 126.2 ng/mL) and the UC group (372.0 ± 196.1 ng/mL) with p < 0.001. With a serum anti-vinculin antibody cutoff value greater than 535.8 ng/mL
(1.8 OD), there is significant diagnostic value in distinguishing between healthy individuals and IBS-D patients, with sensitivity and specificity of 50.0% and 87.5%, respectively.