MỘT SỐ NGUYÊN NHÂN KỸ THUẬT DẪN ĐẾN KẾT QUẢ ÂM TÍNH GIẢ VÀ DƯƠNG TÍNH GIẢ CỦA XÉT NGHIỆM PCR CHẨN ĐOÁN SARS-COV-2

Abstract

Testing, tracing and treating are three main components of Vietnam preventative strategy against Covid-19 pandemic. The testing results, therefore, play a vital role in effectively delineating case-detected area. The PCR method could be considered as a gold standard to confirm the case thanks to its very high sensitivity and specificity. However, many technical aspects may affect the results, leading to false negative and false positive findings. The false negative results may related to inappropriate sample quality, sample collection time or testing reagents. The false positive results might link to manually pipetting errors as well as reagent and instrument contamination. Better identifying such technique issues could improve the intepretation of PCR results and therefore, contribute to laboratory quality control for further pandemic-containing efforts.