TÍNH ĐA DẠNG VÀ ĐỘT BIẾN MẤT GEN HISTIDINE-RICH PROTEIN 2/ 3 TRÊN CÁC PHÂN LẬP Plasmodium falciparum (PfHRP 2/3) TẠI TÂY NGUYÊN VIỆT NAM

Abstract

The objective: To identify the prevalence of pfhrp2/3 deletant P. falciparum isolates circulating within different Highlands provinces.
Subjects and methods: Total of 108 P. falciparum isolates’ DNA was extracted from the dried blood spots (DBS) on filter papers using Chelex-100. Exon 2 of pfhrp2 and pfhrp3 genes were amplified by PCR and detecting the gene deletion by amplifying the highly conserved regions flanking each gene, resolved by agarose gel electrophoresis and visualized under UV documentation light.
Results: In the microscopic analysis of 108 P. falciparum-confirmed samples, but just positive SD-Bioline test of 97.3% (105/108). General molecular analysis revealed the exon 2 of the pfhrp2 gene and amplifying the highly conserved regions was deleted in 3/108 (2.7%) and pfhrp3 gene was deleted in 5/108 (4.6%) in P. falciparum isolates. In which, Pfhrp2 gene deletion proportions were 5.0 % (2/40), 4.5% (1/22), and 0% in Daklak, Dak Nong, and Gia Lai, respectively. None of P.falciparum isolate with double Pfhrp2/Pfhrp3 gene deletion.

Conclusions: This study provides molecular evidence of the existence of P. falciparum isolates lacking the pfhrp2(2.7%) and pfhrp3 genes (4.6%), and their flanking regions. Continuous evaluation of RDTs and molecular surveillance would be recommended to enhance the diagnosis and keep RDT role is one of the most useful tools to malaria diagnosis assistance in remote areas, and where unenough quality microscopy activity.