ĐÁNH GIÁ HIỆU QUẢ CHẨN ĐOÁN BA BỘ KIT REALTIME RT-PCR PHÁT HIỆN SARS-COV-2

Abstract

Introduction: The realtime RT - PCR assay is a standard tool for detecting SARS-CoV-2 being developed by many laboratories and molecular diagnostic companies. The selection of different targeted gene regions resulted in different diagnostic sensitivity.

Objectives: This study aims to compare and evaluate clinical diagnostic performance of three molecular assays for detection of SARS-CoV- 2.

Materials and Methods: A total of 73 clinical specimens collected from patients and suspected cases infected with SARS-CoV-2. Evaluation of diagnostic sensitivity and agreement of three kits including AV - LDA, Realstar SARS-CoV-2 and WHO RT - PCR on LightCycler 480 II. Results: The study results showed the diagnostic sensitivity of 100% for detecting SARS-CoV-2. The comparing results observed a high correlation between kits when analyzed by linear regression and there was no difference in the mean Ct values between the kits by using Bland - Alman plot analyzis.

Conclussion: The study showed that both three molecular assays had the diagnostic sensitivity and clinical performance for detection of SARS-CoV-2 infection. This study provided accurate tools for screening and an effective control of SARS-CoV-2 in epidemic regions.