ĐẶC ĐIỂM KIỂU GEN CỦA CÁC CHỦNG KLEBSIELLA PNEUMONIAE ĐA KHÁNG THUỐC Ở BỆNH NHÂN VÀ MÔI TRƯỜNG KHOA HỒI SỨC TÍCH CỰC BỆNH VIỆN BỆNH NHIỆT ĐỚI TRUNG ƯƠNG

Abstract

Determining the genotype characteristics of multidrug-resistant K. pneumoniae in patients and the ICU environment is essential to apply appropriate treatment, and at the same time identify the source of the bacteria's transmission to effectivelycontrol infection.

Objectives: determine genotype characteristics of multi-drug resistant K. pneumonia by Next Generation Sequencing techniques. Subjects and Methods: sputum, feces, urine and wound pus (if any) collected from the patients and samples collected from the environment around the hospital bed.Prospective, cross-sectional study,  using the NGS technique to sequence the whole genome of K. pneumonia strains in the study.

Results: from 832 K. pneumonia strains collected, 68 STs were identified with the predominance of ST15 (34%) and ST16 (20%). The most common resistance genes were blaNDM (54.45%), blaOXA (46.51%), blaKPC (45.07%), blaCTX (51.80%), blaSHV (98.44%), blaTEM (52.16%). Notable the frequent occurrence of multidrug-resistant K. pneumoniae strains in the patient and/or the ICU environment during the entire study period.

Conclusions: there are some unique STs of K. pneumoniae at the study site. The number of common drug resistance genes is quite large.