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ROLE OF MALDI-TOF MS TECHNIQUE IN IDENTIFICATION OF NONTUBERCULOUS MYCOBACTERIA (NTM)
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Abstract
Objectives: To evaluate the advantages and limitations of MALDI-TOF MS technique in the identification of species and subspecies of Nontuberculous Mycobacteria (NTM).
Subjects and methods: 23 Relevant studies were searched from 2010 to October 2023 through online medical databases including PubMed, Google Scholar, Scopus, and manufacturer documents. The inclusion criteria focused on studies reporting on the effectiveness, sensitivity, specificity, and limitations of the MALDI-TOF MS method for NTM identification.
Results and conclusions: A total of 23 international studies and articles were included in the analysis, consisting of 3 documents related to the MALDI-TOF MS technique and procedural protocols, and 20 documents reporting on NTM identification outcomes using this method. In Vietnam, no studies have
been conducted on the application of MALDI-TOF MS for species or subspecies identification of NTM. Aggregated results indicated that the average rate of high-confidence identification (Score ≥ 1.80) was 88.0% (95% CI: 80.0 - 93.2), whereas reference methods (LPA and/or gene sequencing) achieved concordant
identification in 97.0% of cases (95% CI: 91.5 - 99.4). Identification results by MALDI-TOF MS showed complete concordance (100%) with reference methods for M. chimaera, M. intracellulare, M. gordonae, and the M. fortuitum group. However, there were 3 cases (3%) in which the species could not be identified,
including two M. avium isolates and one M. abscessus complex isolate. Notably, the MALDI-TOF MS technique reduced the identification time by a factor of seven and the cost by threefold compared to other methods. Nevertheless, isolates that are unidentifiable or yield low-confidence scores should be confirmed
by LPA.
Article Details
Keywords
MALDI-TOF MS, Nontuberculous Mycobacteria (NTM), NTM identification
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