ỨNG DỤNG KỸ THUẬT SINH HỌC PHÂN TỬ ĐỂ XÁC ĐỊNH CÁC KIỂU HUYẾT THANH CỦA LIÊN CẦU TAN HUYẾT NHÓM B PHÂN LẬP TỪ ĐƯỜNG SINH DỤC PHỤ NỮ MANG THAI

Abstract

Objectives:The aim of this study is to identified and detect the serotypes of group B Streptococcus isolated from vagina of pregnant women using molecular tools. Subjects and methods: Group B Streptococcus (GBS) were isolated from pregnant women at 35–37 weeks of gestationat the Nghe An Obstetrics and Pediatrics Hospital, Vietnam between May 2018 and July 2019. Overall, 69 clinical isolates of GBS from vaginal swabs of pregnant mothers were identified using the Gram staining, CAMP test and specific PCR. In this study, a multiplex PCR method was used to distinguish between the nine known serotypes of GBS by three reactions.

Results: The results showed that seven serotypes of GBS were identified, including Ia, Ib, II, III, V, VI, and VII. In that, the most prevalent serotypes were III (n = 27/69; 39.13%) and V (n=22/69; 31.89%), followed by serotypes Ia (n=8/69; 11.59%), VI (n=8/69; 11.59%), Ib (n=2/69; 2.90%), II (n=1/69; 1.45%) and VII (n=1/69; 1.45%), respectively. Serotypes IV, VIII and IX were not found.

Conclusions:This is the first study of GBS serotypes was reported from human in Vietnam.Additional investigations should be made to clarify the serotypes of group B Streptococcus in Vietnam.